Tommaso Allan comes in at full-back in place of Mirko Belloni, who looked shell-shocked during last weekend's 66-7 hammering by world champions South Africa.
Exeter Chiefs' Stephen Varney is picked at scrum-half instead of Alessandro Fusco, while Manuel Zuliani and Ross Vintcent will start in the back row in place of Samuele Locatelli and Alessandro Ortombina, who replace them on the bench.
Quesada made five changes in total, with Danilo Fischetti taking over from Muhamed Hasa, who was yellow-carded against the Springboks, at loosehead.
Front rows Gianmarco Lucchesi and Marco Riccioni, and backs Alessandro Garbisi and Federico Mori have been added to the bench.
Argentina coach Felipe Contepomi has also made five changes to his starting XV, although he has lost a lot of experience.
Hooker Bautista Bernasconi comes in for captain Julian Montoya, who suffered a blow to the head in the Pumas' 42-12 defeat to Ireland last week.
Joaquin Moro and Juan Martin Scelzo come into the back row in place of Marcos Kremer, who has an ankle injury, and Juan Martin Gonzalez, who drops to the bench.
Bernasconi and Scelzo have only three caps each, while tighthead Francisco Moreno has four and Moro eight.
Simon Benitez Cruz takes over at scrum-half from Gonzalo Garcia, while Bautista Delguy moves to full-back to cover for Juan Cruz Mallia, who is out of the tournament with a knee sprain, as Rodrigo Isgro comes into the team on the wing.
Forwards Facundo Bosch and Facundo Isa are added to the replacements.
Nations Championship 2026
The inaugural Nations Championship will see six northern hemisphere and six southern hemisphere nations battle it out across six rounds in the July and November international windows before assembling in Twickenham for one unforgettable finals weekend on November 27-29.
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Italy team:
Starters: 15-Tommaso Allan; 14-Ange Capuozzo, 13-Paolo Odogwu, 12-Leonardo Marin, 11-Monty Ioane; 10-Paolo Garbisi, 9-Stephen Varney; 8-Lorenzo Cannone, 7-Manuel Zuliani, 6-Ross Vintcent; 5-Federico Ruzza, 4-Niccolo Cannone; 3-Simone Ferrari, 2-Giacomo Nicotera (capt), 1-Danilo Fischetti
Replacements: 16-Gianmarco Lucchesi, 17-Muhamed Hasa, 18-Marco Riccioni, 19-Enoch Opoku Gyamfi, 20-Alessandro Ortombina, 21-Samuele Locatelli, 22-Alessandro Garbisi, 23-Federico Mori
Argentina team:
Starters: 15-Bautista Delguy, 14-Rodrigo Isgro, 13-Justo Piccardo, 12-Santiago Chocobares, 11-Mateo Carreras, 10-Tomas Albornoz, 9-Simon Benitez Cruz, 8-Juan Martin Scelzo, 7-Joaquin Moro, 6-Pablo Matera, 5-Pedro Rubiolo, 4-Guido Petti, 3-Francisco Moreno, 2-Bautista Bernasconi, 1-Boris Wenger
Replacements: 16-Facundo Bosch, 17-Mayco Vivas, 18-Joel Sclavi, 19-Efraín Elias, 20-Juan Martin Gonzalez, 21-Facundo Isa, 22-Gonzalo Garcia, 23-Geronimo Prisciantelli